Rapid and efficient gene delivery into plant cells using designed peptide carriers.
Manoj Kumar Lakshmanan , Yutaka Kodama , Takeshi Yoshizumi , Kumar Sudesh , and Keiji Numata
Biomacromolecules, Just Accepted Manuscript
DOI: 10.1021/bm301275g
Publication Date (Web): December 6, 2012
Copyright © 2012 American Chemical Society
To develop a new easy and quick gene delivery system for any types of plants, we prepared ionic complexes of plasmid DNA with designed peptide carriers, each of which combined a cell-penetrating peptide (Bp100 or Tat2) with a polycation (nona-arginine or a copolymer of histidine and lysine). The present system via the designed peptides demonstrated rapid and efficient transient transfections into intact leaf cells of Nicotiana benthamiana and Arabidopsis thaliana without protoplast preparations. The designed peptides demonstrated significantly higher transfection efficiency in comparison to the non-fusion peptides (Bp100, Tat2, nona-arginine and copolymer of histidine and lysine), indicating that combination of functional peptides was a key to develop efficient peptide-based gene delivery system. Based on the results, we exhibited the versatility of the designed peptide-based gene delivery system, which will explore the application of plant biotechnology.
Keyword: Peptide Inhibitors